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A universal competitive fluorescence polarization activity assay for S-adenosylmethionine utilizing methyltransferases

A high-throughput, competitive fluorescence polarization immunoassay has been developed for the detection of methyltransferase activity. The assay was designed to detect S-adenosylhomocysteine (AdoHcy), a product of all S-adenosylmethionine (AdoMet)-utilizing methyltransferase reactions. We employed...

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Bibliographic Details
Main Authors: Graves, Tiffany L., Zhang, Yi, Scott, John E.
Format: Artigo
Language:Inglês
Published: 2007
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4144338/
https://ncbi.nlm.nih.gov/pubmed/18028865
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2007.09.025
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