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A ligation-independent cloning technique for high-throughput assembly of transcription activator–like effector genes
Transcription activator–like (TAL) effector proteins derived from Xanthomonas species have emerged as versatile scaffolds for engineering DNA-binding proteins of user-defined specificity and functionality. Here we describe a rapid, simple, ligation-independent cloning (LIC) technique for synthesis o...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4142318/ https://ncbi.nlm.nih.gov/pubmed/23242165 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2460 |
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