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Isolation of human skeletal muscle myosin heavy chain and actin for measurement of fractional synthesis rates

Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), we have developed a simple method to isolate myosin heavy chain (MHC) and actin from small (60–80 mg) human skeletal muscle samples for the determination of their fractional synthesis rates. The amounts of MHC and actin isol...

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Autori principali: HASTEN, D. L., MORRIS, G. S., RAMANADHAM, S., YARASHESKI, K. E.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1998
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4139966/
https://ncbi.nlm.nih.gov/pubmed/9843753
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