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One-step Generation of Phenotype-expressing Triple-knockout Mice with Heritable Mutated Alleles by the CRISPR/Cas9 System

Triple-knockout mice generated by the one-step CRISPR/Cas9 system were examined for the effects of multiple gene modifications on each phenotype and individual gene function. Sixty embryos were transferred, and 9 pups were obtained; all 9 pups had mutations on 3 loci, and 7 pups showed mutations in...

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Hlavní autoři: FUJII, Wataru, ONUMA, Asuka, SUGIURA, Koji, NAITO, Kunihiko
Médium: Artigo
Jazyk:Inglês
Vydáno: The Society for Reproduction and Development 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4139508/
https://ncbi.nlm.nih.gov/pubmed/25110137
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1262/jrd.2013-139
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