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The Sec13p complex and reconstitution of vesicle budding from the ER with purified cytosolic proteins.
SEC13 encodes a 33 kDa protein that participates in vesicle budding from the endoplasmic reticulum (ER). In order to purify a functional form of Sec13p, a SEC13-dihydrofolate reductase (mouse) fusion gene (SEC13:DHFR) was constructed that complements both sec13 temperature sensitive and null mutatio...
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| Gepubliceerd in: | EMBO J |
|---|---|
| Hoofdauteurs: | , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Nature Publishing Group
1993
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC413700/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8223424/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1993.tb06091.x |
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