DegePrime, a Program for Degenerate Primer Design for Broad-Taxonomic-Range PCR in Microbial Ecology Studies

The taxonomic composition of a microbial community can be deduced by analyzing its rRNA gene content by, e.g., high-throughput DNA sequencing or DNA chips. Such methods typically are based on PCR amplification of rRNA gene sequences using broad-taxonomic-range PCR primers. In these analyses, the use...

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Bibliographic Details
Main Authors: Hugerth, Luisa W., Wefer, Hugo A., Lundin, Sverker, Jakobsson, Hedvig E., Lindberg, Mathilda, Rodin, Sandra, Engstrand, Lars, Andersson, Anders F.
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2014
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4135748/
https://ncbi.nlm.nih.gov/pubmed/24928874
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01403-14
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