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Minimization of chloroplast contamination in 16S rRNA gene pyrosequencing of insect herbivore bacterial communities

Chloroplast sequence contamination in 16S ribosomal RNA gene (16S) analyses can be particularly problematic when sampling microbial communities in plants and folivorous arthropods. We previously encountered high levels of plastid contamination in herbivorous insect samples when we used the predomina...

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Bibliografiske detaljer
Main Authors: Hanshew, Alissa S., Mason, Charles J., Raffa, Kenneth F., Currie, Cameron R.
Format: Artigo
Sprog:Inglês
Udgivet: 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4133986/
https://ncbi.nlm.nih.gov/pubmed/23968645
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2013.08.007
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