The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved Nucleotide Analog Interference Probing (trNAIP)

Rho is a ring-shaped, ATP-dependent RNA helicase/translocase that dissociates transcriptional complexes in bacteria. How RNA recognition is coupled to ATP hydrolysis and translocation in Rho is unclear. Here, we develop and use a new combinatorial approach, called time-resolved Nucleotide Analog Int...

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Bibliographische Detailangaben
Hauptverfasser: Soares, Emilie, Schwartz, Annie, Nollmann, Marcello, Margeat, Emmanuel, Boudvillain, Marc
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2014
Schlagworte:
Nucleic Acid Enzymes
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4132721/
https://ncbi.nlm.nih.gov/pubmed/25016524
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku595
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