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In vitro culture and characterization of spermatogonial stem cells on Sertoli cell feeder layer in goat (Capra hircus)
PURPOSE: To develop an efficient protocol for isolation, purification and long-term culture of spermatogonial stem cell (SSC) in goat. METHODS: The isolation of SSC was performed by testicular disaggregation by enzymatic digestion using collagenase IV, trypsin and DNase I. Further SSCs were enriched...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer US
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4130931/ https://ncbi.nlm.nih.gov/pubmed/24958548 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10815-014-0277-1 |
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