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Mitigating Phototoxicity during Multiphoton Microscopy of Live Drosophila Embryos in the 1.0–1.2 µm Wavelength Range

Light-induced toxicity is a fundamental bottleneck in microscopic imaging of live embryos. In this article, after a review of photodamage mechanisms in cells and tissues, we assess photo-perturbation under illumination conditions relevant for point-scanning multiphoton imaging of live Drosophila emb...

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Main Authors: Débarre, Delphine, Olivier, Nicolas, Supatto, Willy, Beaurepaire, Emmanuel
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4128758/
https://ncbi.nlm.nih.gov/pubmed/25111506
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0104250
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