A three-dimensional collagen construct to model lipopolysaccharide-induced activation of BV2 microglia

BACKGROUND: We report a novel method of culturing microglia in three dimension (3D) using collagen as a substrate. By culturing microglia within a matrix, we aim to emulate the physical state of microglia embedded within parenchyma. METHODS: BV2 microglia cell suspensions were prepared with type I c...

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Detalhes bibliográficos
Main Authors: Haw, Randy Tatt Yhew, Tong, Chih Kong, Yew, Andrew, Lee, Han Chung, Phillips, James B, Vidyadaran, Sharmili
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4128540/
https://ncbi.nlm.nih.gov/pubmed/25074682
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1742-2094-11-134
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