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Generation of GFP reporter human induced pluripotent stem cells using AAVS1 safe harbor Transcription Activator-Like Effector Nuclease

Generation of a fluorescent GFP reporter line in human induced pluripotent stem cells (hiPSCs) provides enormous potentials in both basic stem cell research and regenerative medicine. A protocol of efficiently generating such an engineered reporter line by gene targeting is highly desired. Transcrip...

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Autores principales: Luo, Yongquan, Rao, Mahendra, Zou, Jizhong
Formato: Artigo
Lenguaje:Inglês
Publicado: 2014
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4128243/
https://ncbi.nlm.nih.gov/pubmed/24838915
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780470151808.sc05a07s29
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