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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Using recombinant phage as a scaffold to present various protein portions encoded by a directionally cloned cDNA library to immobilized bait molecules is an efficient means to discover interactions. The technique has largely been used to discover protein-protein interactions but the bait molecule to...
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| Główni autorzy: | , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
MyJove Corporation
2014
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4124854/ https://ncbi.nlm.nih.gov/pubmed/24637694 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50685 |
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