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Bottom-up proteome analysis of E. coli using capillary zone electrophoresis-tandem mass spectrometry with an electrokinetic sheath-flow electrospray interface
The E. coli proteome was digested with trypsin and fractionated using solid phase extraction on a C18 SPE column. Seven fractions were collected and analyzed by capillary zone electrophoresis (CZE)-electrospray ionization tandem mass spectrometry (ESI-MS/MS). The separation was performed in a 60 cm...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4121376/ https://ncbi.nlm.nih.gov/pubmed/23798545 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.201300062 |
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