Two β-Galactosidases from the Human Isolate Bifidobacterium breve DSM 20213: Molecular Cloning and Expression, Biochemical Characterization and Synthesis of Galacto-Oligosaccharides

Two β-galactosidases, β-gal I and β-gal II, from Bifidobacterium breve DSM 20213, which was isolated from the intestine of an infant, were overexpressed in Escherichia coli with co-expression of the chaperones GroEL/GroES, purified to electrophoretic homogeneity and biochemically characterized. Both...

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Autors principals: Arreola, Sheryl Lozel, Intanon, Montira, Suljic, Jasmina, Kittl, Roman, Pham, Ngoc Hung, Kosma, Paul, Haltrich, Dietmar, Nguyen, Thu-Ha
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2014
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4121272/
https://ncbi.nlm.nih.gov/pubmed/25089712
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0104056
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