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The Influence of DNA Extraction Procedure and Primer Set on the Bacterial Community Analysis by Pyrosequencing of Barcoded 16S rRNA Gene Amplicons

In this study, the effect of different DNA extraction procedures and primer sets on pyrosequencing results regarding the composition of bacterial communities in the ileum of piglets was investigated. Ileal chyme from piglets fed a diet containing different amounts of zinc oxide was used to evaluate...

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Hlavní autoři: Starke, Ingo C., Vahjen, Wilfried, Pieper, Robert, Zentek, Jürgen
Médium: Artigo
Jazyk:Inglês
Vydáno: Hindawi Publishing Corporation 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4120916/
https://ncbi.nlm.nih.gov/pubmed/25120931
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2014/548683
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