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Measurement of changes in cell volume based on fluorescence quenching

The intracellular fluorescence of 6-methoxy-N-(3-sulfopropyl)- quinolinium (SPQ), a Cl(−)-sensitive fluorescent dye, is quenched by intracellular organic anions and proteins of unknown identity. The concentration of these intracellular quenchers (ICQs), however, is dependent on cell volume. In the a...

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Detalhes bibliográficos
Main Authors: SRINIVAS, S. P., BONANNO, JOSEPH A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2003
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4118695/
https://ncbi.nlm.nih.gov/pubmed/12937987
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00424-003-1145-5
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