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Single-Cell Genome-Wide Bisulfite Sequencing for Assessing Epigenetic Heterogeneity
We report a single-cell bisulfite sequencing method (scBS-Seq) capable of accurately measuring DNA methylation at up to 48.4% of CpGs. We observed that ESCs grown in serum or 2i both display epigenetic heterogeneity, with “2i-like” cells present in serum cultures. In silico integration of 12 individ...
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4117646/ https://ncbi.nlm.nih.gov/pubmed/25042786 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3035 |
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