Radiometal labeling of recombinant proteins by a genetically engineered minimal chelation site: technetium-99m coordination by single-chain Fv antibody fusion proteins through a C-terminal cysteinyl peptide.

We describe a method to facilitate radioimaging with technetium-99m (99mTc) by genetic incorporation of a 99mTc chelation site in recombinant single-chain Fv (sFv) antibody proteins. This method relies on fusion of the sFv C terminus with a Gly4Cys peptide that specifically coordinates 99mTc. By usi...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	George, A J, Jamar, F, Tai, M S, Heelan, B T, Adams, G P, McCartney, J E, Houston, L L, Weiner, L M, Oppermann, H, Peters, A M
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1995
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC41156/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7667295/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.18.8358
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