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A quantitative method to identify microRNAs targeting a messenger RNA using a 3′UTR RNA affinity technique

The identification of specific microRNAs (miRNAs) that target a given messenger RNA (mRNA) is essential for studies in gene regulation, but the available bioinformatic software programs are often unreliable. We have developed a unique experimental miRNA affinity assay whereby a 3′UTR RNA is end-labe...

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: Shi, Miao, Han, Weiguo, Spivack, Simon D.
Format: Artigo
Langue:Inglês
Publié: 2013
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4112567/
https://ncbi.nlm.nih.gov/pubmed/23938772
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.08.002
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