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Spin labeling of a cysteine residue of the Escherichia coli outer membrane lipoprotein in its membrane environment

A method was developed to attach a spin label to a specific site on the structural lipoprotein of the Escherichia coli outer membrane in situ. This method takes advantage of the fact that the outer membrane of wild-type E. coli contains few residues reactive towards sulfhydryl reagents. A mutant E....

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Lee, Nancy, Scandella, Carl, Inouye, Masayori
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1978
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC411197/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/203927/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.1.127
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