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How a Replication Origin and Matrix Attachment Region Accelerate Gene Amplification under Replication Stress in Mammalian Cells

The gene amplification plays a critical role in the malignant transformation of mammalian cells. The most widespread method for amplifying a target gene in cell culture is the use of methotrexate (Mtx) treatment to amplify dihydrofolate reductase (Dhfr). Whereas, we found that a plasmid bearing both...

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Bibliografiska uppgifter
Huvudupphovsmän: Tanaka, Shun-suke, Mitsuda, Sho-hei, Shimizu, Noriaki
Materialtyp: Artigo
Språk:Inglês
Publicerad: Public Library of Science 2014
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4111587/
https://ncbi.nlm.nih.gov/pubmed/25061979
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0103439
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