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Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
This article describes a method to detect and analyze dynamic interactions between a protein of interest and other factors in vivo. Our method is based on the amber suppression technology that was originally developed by Peter Schultz and colleagues(1). An amber mutation is first introduced at a spe...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
MyJove Corporation
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4111254/ https://ncbi.nlm.nih.gov/pubmed/24378574 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51217 |
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