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EQUILIBRIUM AND DYNAMIC DESIGN PRINCIPLES FOR BINDING MOLECULES ENGINEERED FOR REAGENTLESS BIOSENSORS

Reagentless biosensors rely on the interaction of a binding partner and its target to generate a change in fluorescent signal using an environment sensitive fluorophore or Förster Resonance Energy Transfer. Binding affinity can exert a significant influence on both the equilibrium and the dynamic re...

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Detalles Bibliográficos
Autores principales: de Picciotto, Seymour, Imperiali, Barbara, Griffith, Linda G., Wittrup, K. Dane
Formato: Artigo
Lenguaje:Inglês
Publicado: 2014
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4104283/
https://ncbi.nlm.nih.gov/pubmed/24814226
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2014.04.036
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