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Long-Term Culture of Rat Hippocampal Neurons at Low Density in Serum-Free Medium: Combination of the Sandwich Culture Technique with the Three-Dimensional Nanofibrous Hydrogel PuraMatrix
The primary culture of neuronal cells plays an important role in neuroscience. There has long been a need for methods enabling the long-term culture of primary neurons at low density, in defined serum-free medium. However, the lower the cell density, the more difficult it is to maintain the cells in...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
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Public Library of Science
2014
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4102532/ https://ncbi.nlm.nih.gov/pubmed/25032834 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0102703 |
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