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Quantitative Metabolomics and Instationary (13)C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris
Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary (13)C metabolic flux analysis (INST (13)C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impact of the production of a Rhizopus oryzae lipase (...
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| Autors principals: | , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
MDPI
2014
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4101507/ https://ncbi.nlm.nih.gov/pubmed/24957027 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/metabo4020281 |
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