Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

Psychrophilic basidiomycete yeast, Glaciozyma antarctica strain PI12, was shown to be a protease-producer. Isolation of the PI12 protease gene from genomic and mRNA sequences allowed determination of 19 exons and 18 introns. Full-length cDNA of PI12 protease gene was amplified by rapid amplification...

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Bibliographische Detailangaben
Hauptverfasser: Alias, Norsyuhada, Ahmad Mazian, Mu'adz, Salleh, Abu Bakar, Basri, Mahiran, Rahman, Raja Noor Zaliha Raja Abd.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Hindawi Publishing Corporation 2014
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4100274/
https://ncbi.nlm.nih.gov/pubmed/25093119
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2014/197938
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