A Cautionary Note on the Use of Split-YFP/BiFC in Plant Protein-Protein Interaction Studies

Since its introduction in plants 10 years ago, the bimolecular fluorescence complementation (BiFC) method, or split-YFP (yellow fluorescent protein), has gained popularity within the plant biology field as a method to study protein-protein interactions. BiFC is based on the restoration of fluorescen...

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Bibliographic Details
Main Authors: Horstman, Anneke, Nougalli Tonaco, Isabella Antonia, Boutilier, Kim, Immink, Richard G. H.
Format: Artigo
Language:Inglês
Published: MDPI 2014
Subjects:
Technical Note
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4100113/
https://ncbi.nlm.nih.gov/pubmed/24886811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms15069628
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