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Visualization of Phosphatidic Acid Fluctuations in the Plasma Membrane of Living Cells
We developed genetically-encoded fluorescent sensors based on Förster Resonance Energy Transfer to monitor phosphatidic acid (PA) fluctuations in the plasma membrane using Spo20 as PA-binding motif. Basal PA levels and phospholipase D activity varied in different cell types. In addition, stimuli tha...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4099201/ https://ncbi.nlm.nih.gov/pubmed/25025521 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0102526 |
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