Imaging single membrane fusion events mediated by SNARE proteins

Using total internal reflection fluorescence microscopy, we have developed an assay to monitor individual fusion events between proteoliposomes containing vesicle soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and a supported planar bilayer containing cognate target...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Fix, Marina, Melia, Thomas J., Jaiswal, Jyoti K., Rappoport, Joshua Z., You, Daoqi, Söllner, Thomas H., Rothman, James E., Simon, Sanford M.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2004
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC409915/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15123811/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.0401779101
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