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Mechanical Homogenization Increases Bacterial Homogeneity in Sputum

Sputum obtained from patients with cystic fibrosis (CF) is highly viscous and often heterogeneous in bacterial distribution. Adding dithiothreitol (DTT) is the standard method for liquefaction prior to processing sputum for molecular detection assays. To determine if DTT treatment homogenizes the ba...

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Detalhes bibliográficos
Main Authors: Stokell, Joshua R., Khan, Ammad, Steck, Todd R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4097738/
https://ncbi.nlm.nih.gov/pubmed/24759710
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00487-14
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