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Mechanical Homogenization Increases Bacterial Homogeneity in Sputum

Sputum obtained from patients with cystic fibrosis (CF) is highly viscous and often heterogeneous in bacterial distribution. Adding dithiothreitol (DTT) is the standard method for liquefaction prior to processing sputum for molecular detection assays. To determine if DTT treatment homogenizes the ba...

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Detalles Bibliográficos
Autores principales: Stokell, Joshua R., Khan, Ammad, Steck, Todd R.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2014
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4097738/
https://ncbi.nlm.nih.gov/pubmed/24759710
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00487-14
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