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Heritable Genome Editing with CRISPR/Cas9 in the Silkworm, Bombyx mori

We report the establishment of an efficient and heritable gene mutagenesis method in the silkworm Bombyx mori using modified type II clustered regularly interspaced short palindromic repeats (CRISPR) with an associated protein (Cas9) system. Using four loci Bm-ok, BmKMO, BmTH, and Bmtan as candidate...

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Main Authors: Wei, Wei, Xin, Huhu, Roy, Bhaskar, Dai, Junbiao, Miao, Yungen, Gao, Guanjun
格式: Artigo
語言:Inglês
出版: Public Library of Science 2014
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4094479/
https://ncbi.nlm.nih.gov/pubmed/25013902
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0101210
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