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Heritable Genome Editing with CRISPR/Cas9 in the Silkworm, Bombyx mori

We report the establishment of an efficient and heritable gene mutagenesis method in the silkworm Bombyx mori using modified type II clustered regularly interspaced short palindromic repeats (CRISPR) with an associated protein (Cas9) system. Using four loci Bm-ok, BmKMO, BmTH, and Bmtan as candidate...

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Detalhes bibliográficos
Main Authors: Wei, Wei, Xin, Huhu, Roy, Bhaskar, Dai, Junbiao, Miao, Yungen, Gao, Guanjun
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4094479/
https://ncbi.nlm.nih.gov/pubmed/25013902
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0101210
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