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Super-Resolution Microscopy Using Standard Fluorescent Proteins in Intact Cells under Cryo-Conditions

[Image: see text] We introduce a super-resolution technique for fluorescence cryo-microscopy based on photoswitching of standard genetically encoded fluorescent marker proteins in intact mammalian cells at low temperature (81 K). Given the limit imposed by the lack of cryo-immersion objectives, curr...

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Detalhes bibliográficos
Main Authors: Kaufmann, Rainer, Schellenberger, Pascale, Seiradake, Elena, Dobbie, Ian M., Jones, E. Yvonne, Davis, Ilan, Hagen, Christoph, Grünewald, Kay
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2014
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4092024/
https://ncbi.nlm.nih.gov/pubmed/24884378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/nl501870p
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