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Tissue Optical Clearing, Three-Dimensional Imaging, and Computer Morphometry in Whole Mouse Lungs and Human Airways

In whole adult mouse lung, full identification of airway nerves (or other cellular/subcellular objects) has not been possible due to patchy distribution and micron-scale size. Here we describe a method using tissue clearing to acquire the first complete image of three-dimensional (3D) innervation in...

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Detalhes bibliográficos
Main Authors: Scott, Gregory D., Blum, Emily D., Fryer, Allison D., Jacoby, David B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Thoracic Society 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4091855/
https://ncbi.nlm.nih.gov/pubmed/24471696
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1165/rcmb.2013-0284OC
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