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Recombinant production, crystallization and X-ray crystallographic structure determination of peptidyl-tRNA hydrolase from Salmonella typhimurium

Peptidyl-tRNA hydrolase (Pth; EC 3.1.1.29) from the pathogenic bacterium Salmonella typhimurium has been cloned, expressed in Escherichia coli and crystallized for X-ray analysis. Crystals were grown using hanging-drop vapor diffusion against a reservoir solution consisting of 0.03 M citric acid, 0....

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Detalhes bibliográficos
Main Authors: Vandavasi, Venugopal, Taylor-Creel, Kasey, McFeeters, Robert L., Coates, Leighton, McFeeters, Hana
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4089523/
https://ncbi.nlm.nih.gov/pubmed/25005080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14009893
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