Proteomic Analysis of Intact Flagella of Procyclic Trypanosoma brucei Cells Identifies Novel Flagellar Proteins with Unique Sub-localization and Dynamics

Cilia and flagella are complex organelles made of hundreds of proteins of highly variable structures and functions. Here we report the purification of intact flagella from the procyclic stage of Trypanosoma brucei using mechanical shearing. Structural preservation was confirmed by transmission elect...

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Detalhes bibliográficos
Main Authors: Subota, Ines, Julkowska, Daria, Vincensini, Laetitia, Reeg, Nele, Buisson, Johanna, Blisnick, Thierry, Huet, Diego, Perrot, Sylvie, Santi-Rocca, Julien, Duchateau, Magalie, Hourdel, Véronique, Rousselle, Jean-Claude, Cayet, Nadège, Namane, Abdelkader, Chamot-Rooke, Julia, Bastin, Philippe
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2014
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4083114/
https://ncbi.nlm.nih.gov/pubmed/24741115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M113.033357
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