Combined enrichment of neuromelanin granules and synaptosomes from human substantia nigra pars compacta tissue for proteomic analysis

This article gives a detailed description of a protocol using density gradient centrifugation for the enrichment of neuromelanin granules and synaptosomes from low amounts (≥0.15 g) of human substantia nigra pars compacta tissue. This has a great advantage compared to already existing methods as it...

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Bibliografiske detaljer
Main Authors: Plum, S., Helling, S., Theiss, C., Leite, R.E.P., May, C., Jacob-Filho, W., Eisenacher, M., Kuhlmann, K., Meyer, H.E., Riederer, P., Grinberg, L.T., Gerlach, M., Marcus, K.
Format: Artigo
Sprog:Inglês
Udgivet: 2013
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4083096/
https://ncbi.nlm.nih.gov/pubmed/23917253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jprot.2013.07.015
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