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Single-stranded DNA as a Cleavable Linker for Bioorthogonal Click Chemistry-based Proteomics
In this report, we present a new class of cleavable linker based on automatically synthesized, single-stranded DNAs. We incorporated a DNA oligo into an azide-functionalized biotin (biotin-DNA-N(3)) and used the probe to enrich alkyne-tagged glycoproteins from mammalian cell lysates. Highly efficien...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4078914/ https://ncbi.nlm.nih.gov/pubmed/23627610 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc400093x |
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