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Proteomic profiling of tandem affinity purified 14-3-3 protein complexes in Arabidopsis thaliana

In eukaryotes, 14-3-3 dimers regulate hundreds of functionally diverse proteins (clients), typically in phosphorylation-dependent interactions. To uncover new clients, a 14-3-3 omega (At1g78300) from Arabidopsis was engineered with a “tandem affinity purification” (TAP) tag and expressed in transgen...

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Detalhes bibliográficos
Main Authors: Chang, Ing-Feng, Curran, Amy, Woolsey, Rebekah, Quilici, David, Cushman, John, Mittler, Ron, Harmon, Alice, Harper, Jeffrey
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4077669/
https://ncbi.nlm.nih.gov/pubmed/19452453
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.200800445
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