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Single-cell western blotting

To measure cell-to-cell variation in protein-mediated functions — a hallmark of biological processes — we developed an approach to conduct ~10(3) concurrent single-cell western blots (scWesterns) in ~4 hours. A microscope slide supporting a 30 µm-thick photoactive polyacrylamide gel enables western...

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Bibliografiske detaljer
Main Authors: Hughes, Alex J., Spelke, Dawn P., Xu, Zhuchen, Kang, Chi-Chih, Schaffer, David V., Herr, Amy E.
Format: Artigo
Sprog:Inglês
Udgivet: 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4077215/
https://ncbi.nlm.nih.gov/pubmed/24880876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2992
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