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A real-time high-throughput fluorescence assay for sphingosine kinases
Sphingosine kinases (SphKs), of which there are two isoforms, SphK1 and SphK2, have been implicated in regulation of many important cellular processes. We have developed an assay for monitoring SphK1 and SphK2 activity in real time without the need for organic partitioning of products, radioactive m...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The American Society for Biochemistry and Molecular Biology
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4076073/ https://ncbi.nlm.nih.gov/pubmed/24792926 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D048132 |
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