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A real-time high-throughput fluorescence assay for sphingosine kinases

Sphingosine kinases (SphKs), of which there are two isoforms, SphK1 and SphK2, have been implicated in regulation of many important cellular processes. We have developed an assay for monitoring SphK1 and SphK2 activity in real time without the need for organic partitioning of products, radioactive m...

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Detalhes bibliográficos
Main Authors: Lima, Santiago, Milstien, Sheldon, Spiegel, Sarah
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4076073/
https://ncbi.nlm.nih.gov/pubmed/24792926
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D048132
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