Highly automated nano-LC/MS-based approach for thousand cell-scale quantification of side chain-hydroxylated oxysterols

Iso-octyl chain-hydroxylated oxysterols were determined in attomoles per 10,000 cells concentrations in 10,000–80,000 cultured pancreatic adenocarcinoma cells, using a sensitive, highly automated nano-LC-ESI-MS-based method. Identified oxysterols included 24S hydroxycholesterol (24S-OHC), 25 hydroxy...

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Autori principali: Roberg-Larsen, Hanne, Lund, Kaja, Vehus, Tore, Solberg, Nina, Vesterdal, Caroline, Misaghian, Dorna, Olsen, Petter Angell, Krauss, Stefan, Wilson, Steven Ray, Lundanes, Elsa
Natura: Artigo
Lingua:Inglês
Pubblicazione: The American Society for Biochemistry and Molecular Biology 2014
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4076067/
https://ncbi.nlm.nih.gov/pubmed/24792927
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D048801
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