Trypan blue exclusion assay by flow cytometry

Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be...

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Autors principals: Avelar-Freitas, B.A., Almeida, V.G., Pinto, M.C.X., Mourão, F.A.G., Massensini, A.R., Martins-Filho, O.A., Rocha-Vieira, E., Brito-Melo, G.E.A.
Format: Artigo
Idioma:Inglês
Publicat: Associação Brasileira de Divulgação Científica 2014
Matèries:
Biomedical Sciences
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4075294/
https://ncbi.nlm.nih.gov/pubmed/24652322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/1414-431X20143437
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