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Quantitative, Label-Free Characterization of Stem Cell Differentiation at the Single-Cell Level by Broadband Coherent Anti-Stokes Raman Scattering Microscopy

We use broadband coherent anti-Stokes Raman scattering (BCARS) microscopy to characterize lineage commitment of individual human mesenchymal stem cells cultured in adipogenic, osteogenic, and basal culture media. We treat hyperspectral images obtained by BCARS in two independent ways, obtaining robu...

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Autors principals: Lee, Young Jong, Vega, Sebastián L., Patel, Parth J., Aamer, Khaled A., Moghe, Prabhas V., Cicerone, Marcus T.
Format: Artigo
Idioma:Inglês
Publicat: Mary Ann Liebert, Inc. 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4074749/
https://ncbi.nlm.nih.gov/pubmed/24224876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/ten.tec.2013.0472
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