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An Optimized Protocol for Isolating Primary Epithelial Cell Chromatin for ChIP
A critical part of generating robust chromatin immunoprecipitation (ChIP) data is the optimization of chromatin purification and size selection. This is particularly important when ChIP is combined with next-generation sequencing (ChIP-seq) to identify targets of DNA-binding proteins, genome-wide. C...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4074041/ https://ncbi.nlm.nih.gov/pubmed/24971909 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0100099 |
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