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Expression of Recombinant Human FADD, Preparation of Its Polyclonal Antiserum and the Application in Immunoassays
The wild-type human Fas-associated death domain (FADD) protein was expressed as a His-tag fusion protein in Escherichia coli. Recombinant FADD proteins were purified under the denatured condition. After denatured protein purification, it was refolded and obtained at a yield of about 23 mg/L. Purifie...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4073656/ https://ncbi.nlm.nih.gov/pubmed/19118514 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/cmi.2008.59 |
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