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Inhibition of Protein Carbamylation in Urea Solution Using Ammonium Containing Buffers
Urea solution is one of the most commonly employed protein denaturants for protease digestion in proteomic studies. However, it has long been recognized that urea solution can cause carbamylation at the N-termini of proteins/peptides and at the side chain amino groups of lysine and arginine residues...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2013
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4072244/ https://ncbi.nlm.nih.gov/pubmed/24161613 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.10.024 |
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