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Imaging the fate of histone Cse4 reveals de novo replacement in S phase and subsequent stable residence at centromeres
The budding yeast centromere contains Cse4, a specialized histone H3 variant. Fluorescence pulse-chase analysis of an internally tagged Cse4 reveals that it is replaced with newly synthesized molecules in S phase, remaining stably associated with centromeres thereafter. In contrast, C-terminally-tag...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
eLife Sciences Publications, Ltd
2014
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4067749/ https://ncbi.nlm.nih.gov/pubmed/24844245 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.02203 |
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