Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells

The ability to edit the genome is essential for many state-of-the-art experimental paradigms. Since DNA breaks stimulate repair, they can be exploited to target site-specific integration. The clustered, regularly interspaced, short palindromic repeats (CRISPR)/cas9 system from Streptococcus pyogenes...

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Bibliographic Details
Main Authors: Böttcher, Romy, Hollmann, Manuel, Merk, Karin, Nitschko, Volker, Obermaier, Christina, Philippou-Massier, Julia, Wieland, Isabella, Gaul, Ulrike, Förstemann, Klaus
Format: Artigo
Language:Inglês
Published: Oxford University Press 2014
Subjects:
Genome Integrity, Repair and Replication
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4066747/
https://ncbi.nlm.nih.gov/pubmed/24748663
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku289
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