Mass spectrometry of membrane transporters reveals subunit stoichiometry and interactions

We describe a general mass spectrometry approach to determine subunit stoichiometry and lipid binding in intact membrane protein complexes. By exploring conditions for preserving interactions during transmission into the gas phase and for optimally stripping away detergent, by subjecting the complex...

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Detalhes bibliográficos
Main Authors: Barrera, Nelson P, Isaacson, Shoshanna C, Zhou, Min, Bavro, Vassiliy N, Welch, Alex, Schaedler, Theresia A, Seeger, Markus A, Miguel, Ricardo Núñez, Korkhov, Vladimir M, van Veen, Hendrik W, Venter, Henrietta, Walmsley, Adrian R, Tate, Christopher G, Robinson, Carol V
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4066579/
https://ncbi.nlm.nih.gov/pubmed/19578383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1347
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